YAP (D8H1X) XP® Rabbit mAb #14074:
为了获得最佳的 ChIP 和 ChIP-seq 结果，每次免疫沉淀使用 10 μl 抗体和 10 μg 染色质（大约 4 x 106 个细胞）。该抗体已使用 SimpleChIP® Enzymatic Chromatin IP Kits 进行了验证。
|
应用
|
稀释度
|
|
蛋白质印迹法
|
1:1000
|
|
免疫沉淀
|
1:50
|
|
免疫组织化学（石蜡）
|
1:400
|
|
免疫荧光法（免疫细胞化学）
|
1:100
|
|
流式细胞术
|
1:50
|
|
染色质免疫沉淀法
|
1:50
|
|
ChIP-seq
|
1:50
|
存储：
保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。存储温度为 -20℃。请不要分装抗体。
相关产品
-
6-Tube Magnetic Separation Rack
-
Anti-biotin, HRP-linked Antibody
-
Anti-rabbit IgG, HRP-linked Antibody
-
Biotinylated Protein Ladder Detection Pack
-
ChIP-Grade Protein G Agarose Beads
-
ChIP-Grade Protein G Magnetic Beads
-
Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb
-
Normal Goat Serum
-
Phospho-YAP (Ser109) (E5I9G) Rabbit mAb
-
Phospho-YAP (Ser109) Antibody
-
Phospho-YAP (Ser127) (D9W2I) Rabbit mAb
-
Phospho-YAP (Ser127) Antibody
-
Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb
-
Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
-
SignalStain® Antibody Diluent
-
SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
-
SignalStain® DAB Substrate Kit
-
SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
-
SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
-
SimpleChIP® Human CTGF Promoter Primers
-
SimpleChIP® Human CTGF Upstream Primers
-
SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
-
SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
-
Tris Buffered Saline with Tween® 20 (TBST-10X)
